Journal: Cell reports

Article Title: Microglia degrade Alzheimer’s amyloid-beta deposits extracellularly via digestive exophagy

doi: 10.1016/j.celrep.2024.115052

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The pellet was resuspended in complete DMEM, triturated with a 10 mL serological pipette, and then transferred to a vented T75 tissue culture-treated flask (Corning, pn# 353136) and incubated in a humidified cell incubator at 37°C with 5% CO 2 for two days.

Techniques: Recombinant, Cell Culture, Electron Microscopy, Software, Polymer, Microscopy, Spectrophotometry, Membrane, Transmission Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains

doi: 10.3389/fbioe.2020.582650

Figure Lengend Snippet: Introduction to minibrains. (A) Schematics show the timeline of processes involved in minibrain development. (B) Shows 12-week-old minibrains in a 6-well plate. (C) Volume rendered image of Golgi-Cox and DAPI stained (a nuclear stain) 12-week-old whole minibrain showing neurons with elaborate projections (color coded in green) and cells with short protrusions (color coded in pink) (see ). (D) Shows microtome cut 12-week-old minibrain section stained with GFAP, a marker of glial cells and DAPI. (E) Shows volume rendered image of viral labeled 12-week-old whole minibrain showing tdTomato (a fluorescent protein) labeled projection neurons. W, Week of minibrain generation.

Article Snippet: 6-well culture plate (Greiner Bio-One, #657 160) 96-well culture plate for imaging (Greiner bio-one, #655090) 384 well culture plate for imaging (Greiner bio-one, #781091) 24 well culture plate non-treated (Nunc, #144530) Breathable plate sealer (Greiner Bio-One, #676051) 25 cm 2 cell culture flask (T25) (Corning Falcon, #353109) 75 cm 2 cell culture flask (T75) Flask (Corning Falcon, #353136) 175 cm 2 cell culture flask (T175) Flask (Corning Falcon, #353112) Sterile Hydrophilic PTFE membrane for tissue cultures, 2 mm diameter (named as « confetti ») (PTFE-005, HEPIA Biosciences) Cell culture inserts for 6-well plate (Merck Millipore, #044003) 0.2 ml PCR tube (Thermoscientific, #AB-0784) 1.5 ml microfuge tubes (Eppendorf, #0030125150) 15 ml tube with conical bottom (Corning Falcon, #352096) 50 ml tube with conical bottom (Corning Falcon, #352070) Pointe 10–20 μl cleanpack clearline sterile (Milian, #010320) Pointe 20 μl cleanpack clearline sterile (Milian, #713178) Pointe 200 μl cleanpack clearline sterile (Milian, #713179) Pointe 1,000 μl cleanpack clearline sterile (Milian, #713180) 10 μl pipette (Rainin, #17014388) 20 μl pipette (Rainin, #17014392) 200 μl pipette (Rainin, #17014391) 1,000 μl pipette (Rainin, #17014382) 2 ml sterile aspirating pipets (Corning Falcon, #357558) 12 mm glass coverslips (SPL Life Sciences, #20012) Luna Cell Counting Slides (Logos biosystems, #L12001) Sample holder (HEPIA, ).

Techniques: Staining, Marker, Labeling

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains

doi: 10.3389/fbioe.2020.582650

Figure Lengend Snippet: Size and number of minibrains generated using the protocol. (A) Shows the distribution of size of minibrains older than 8 weeks across three different batches (N1, N2, and N3) (with seeding density of 2.5e5 cells per well) as a whisker plot. Mean ± Standard deviation (SD) of N1, N2, and N3 are 546.80 ± 62.40, 623.61 ± 68.52 and 584.73 ± 79.02, respectively. Collective mean ± SD of all the three batches is 550.64 ± 75.19. N1 = 199 minibrains, N2 = 304 minibrains, N3 = 93 minibrains. (B) Shows the number of minibrains (more than 8 weeks old) generated per well in a 6-well plate across three different batches (N1, N2 and N3 with seeding density of 2.5E5 cells per well) as a whisker plot. The number of minibrains generated across three batches are not significantly different ( p = 0.02 8) as per Kruskal Wallis statistical test. Mean ± SD of N1, N2, and N3 are 42.93 ± 12.00, 47.75 ± 8.37, 52.47 ± 16.38, respectively. Collective mean ± SD all the three batches is 48.97 ± 12.68. N1 = 15 wells, N2 = 45 wells and N3 = 60 wells.

Article Snippet: 6-well culture plate (Greiner Bio-One, #657 160) 96-well culture plate for imaging (Greiner bio-one, #655090) 384 well culture plate for imaging (Greiner bio-one, #781091) 24 well culture plate non-treated (Nunc, #144530) Breathable plate sealer (Greiner Bio-One, #676051) 25 cm 2 cell culture flask (T25) (Corning Falcon, #353109) 75 cm 2 cell culture flask (T75) Flask (Corning Falcon, #353136) 175 cm 2 cell culture flask (T175) Flask (Corning Falcon, #353112) Sterile Hydrophilic PTFE membrane for tissue cultures, 2 mm diameter (named as « confetti ») (PTFE-005, HEPIA Biosciences) Cell culture inserts for 6-well plate (Merck Millipore, #044003) 0.2 ml PCR tube (Thermoscientific, #AB-0784) 1.5 ml microfuge tubes (Eppendorf, #0030125150) 15 ml tube with conical bottom (Corning Falcon, #352096) 50 ml tube with conical bottom (Corning Falcon, #352070) Pointe 10–20 μl cleanpack clearline sterile (Milian, #010320) Pointe 20 μl cleanpack clearline sterile (Milian, #713178) Pointe 200 μl cleanpack clearline sterile (Milian, #713179) Pointe 1,000 μl cleanpack clearline sterile (Milian, #713180) 10 μl pipette (Rainin, #17014388) 20 μl pipette (Rainin, #17014392) 200 μl pipette (Rainin, #17014391) 1,000 μl pipette (Rainin, #17014382) 2 ml sterile aspirating pipets (Corning Falcon, #357558) 12 mm glass coverslips (SPL Life Sciences, #20012) Luna Cell Counting Slides (Logos biosystems, #L12001) Sample holder (HEPIA, ).

Techniques: Generated, Whisker Assay, Standard Deviation